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St. John Health System
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Index:

Platelet Autoantibody

Order Name PLT AUTOAB
Test Number: 5577375
Revision Date 01/06/2020
Test Name Methodology LOINC Code
Platelet Autoantibody
Enzyme-Linked Immunosorbent Assay (ELISA) 24374-1 
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 40 mL (10 mL) Whole Blood ACD Solution A (Yellow Top) Refrigerated
Instructions Due to specimen integrity and stability reasons, this must be collected Monday through Thursday..!
Do not collect befor a weekend or a Holiday


40 mL required for Platelet Count < 100,000
10 mL required for Platelet Count > 100,000
Keep specimen Refrigerated!

Specimen Must Be Received at the Reference Lab within 4 days of collection.
Specimen stability: Ambient Unacceptable, Refrigerated 4 days, Frozen Unacceptable.

No alternative specimen types are accepted for this test per the Reference Lab
GENERAL INFORMATION
Testing Schedule Varies 
Expected TAT 3-5 Days 
Clinical Use The platelet autoantibody study is designed to detect platelet autoantibodies eluted from the patient’s platelets or circulating in the patient's serum or plasma directed against GPIIb/IIIa, GPIb/IX, and GPIa/IIa. These antibodies can be detected in patients with autoimmune thrombocytopenic purpura (ITP or AITP). This test is intended to help identify patients who present with unexplained thrombocytopenia that is secondary to immune destruction. A positive test is considered diagnostic, while a negative test does not rule out the diagnosis. Repeat testing can sometimes be of benefit. 
Notes Testing Performed At: The BloodCenter of Wisconsin
Test Code: 5544
CPT Code(s) 86022, 86023
Internal Comments 11/13/14:  The following sentence was removed from instructions at request of Mary Pille:
All Collections Must Be Scheduled for Collection! Contact: Immunology (918)744-2553 x15511 or Immunology Manager x15788.
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5/17/17 changed collection info from "Due to specimen integrity and stability reasons, it is highly suggested this should be collected at the RML Main Laboratory - Monday through Thursday..!"  to "Due to specimen integrity and stability issues, must be drawn only on Monday-Thursday"  per Patti L - JK
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10/23/17 This is what was previously in the specimen instructions JK:
--- begin of old text
Due to specimen integrity and stability reasons, this must be collected Monday through Thursday..!
Testing must begin within 24hrs.

Please make this a Separate Specimen, Do not share this specimen with other testing.
Keep specimen as Whole Blood and On-ICE..! Do Not Centrifuge!
Transport specimen directly to Lab Section ASAP On-ICE but Do Not Freeze!

(Note: Special processing of the sample will be performed within the performing laboratory section. Hemolyzed, Icteric and Lipemic specimens will yield false results and will not be tested.)
--- end of old text
10/15/18 moved to Blood Center of Wiscnonsin. - JK

01/07/2019: Removed alternate specimen type EDTA (Ticket # 656820) - SN
01/11/2019: Updated specimen type (Ticket 657437) - SNN
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3/14/19 added Must to specimen delivery instructions - JK
1-6-2020 CPT change from 86022 to 86022, 86023
Service Provided By Labcorp Oklahoma, Inc.
Lab Section Reference Lab