ACTH (ADRENOCORTICOTROPIC HORMONE) STIMULATION

Index:

SJHS Orderable Tests Only

Order Name ACTH STIM
Test Number: 2002151
Revision Date 10/01/2022

Test Name	Methodology	LOINC Code
Cortisol Baseline	Chemiluminescent Micropartical Immunoassay	43215-3
Cortisol 30 Minute Specimen	Chemiluminescent Micropartical Immunoassay	12557-5
Cortisol 60 Minute Specimen	Chemiluminescent Micropartical Immunoassay	12558-3

SPECIMEN REQUIREMENTS
Specimen	Specimen Volume (min)	Specimen Type	Specimen Container	Transport Environment
Preferred	1 mL (0.5)	Serum	Clot Activator SST	Refrigerated or Frozen
Alternate 1	1 mL (0.5)	Plasma	Lithium Heparin PST (Light Green Top)	Refrigerated or Frozen
Instructions	This test needs to be scheduled for Collection. See information in the Notes Section below. Stability: Ambient 1 day. Refrigerated 14 days. Frozen 30 days.

GENERAL INFORMATION

Testing Schedule

Mon - Fri

Expected TAT

1-3 days

Clinical Use

This test determines how the adrenal glands respond to a certain hormone. The hormone is called adrenocorticotrophic hormone, also known as ACTH. ACTH is made in the pituitary gland and travels through the bloodstream to the adrenal glands. ACTH stimulates the adrenal gland to release Cortisol. Cortisol is a key factor in many functions in the body's metabolism of fats, carbohydrates, sodium, potassium, and protein.

Notes

Testing Locations
Tulsa	Cortisol (ACTH) Stimulations may be scheduled with Labcorp Oklahoma, Inc. Patient Service Center at St. John Medical Center for collections M-F 7:30am to 4:00pm at (918) 744-3131 x 15531; after 4:00pm may be scheduled with Chemistry department at (918)744-3131 x 15514. Testing is performed Monday through Friday 9:00am to 11am. and requires about 1 hour and 15 minutes of the patient's time.
Bartlesville	Cortisol (ACTH) stimulations may be scheduled with Labcorp Oklahoma, Inc. at Jane Phillips Medical Center, Monday through Friday at (918) 331-1737.
Physician Office	If the testing is performed in the physician's office, the following protocol is suggested.
ACTH Stimulation Test
Baseline Cortisol - Have a Cortisol drawn prior to injection (5 ml gold top tube). Note the time drawn on the tube. Administer 250 micrograms (1 vial) of synthetic ACTH Cortrosyn (Cosyntropin) intravenously. Use supplied diluents to dissolve ACTH powder. Inject over 1-2 minutes. 30 minute Cortisol - draw one clot tube for a Cortisol level 30 minutes after the injection. Note the time drawn on the tube. 60 minute Cortisol - draw a second clot tube for a Cortisol level 60 minutes after the injection. Note the time drawn on the tube.

CPT Code(s)

80400, 82533, 80503

Internal Comments

11/12/15 changed cpt 82533x3, 80500 to 80400, 82533, 80500 - per Leilani H.
3/9/17 changed call in number from Please Call RML Chemistry department (918) 744-2500 x15514 to schedule collection to Misty Poletek @ (918) 744-2500 ext 15531 - JK
10/26/17 changing scheduling text from
This test needs to be scheduled for Collection. Please Call Misty Poletek (918) 744-2500 x15531 to schedule collection. Please refer to this test in our Specialized Tests section of our website for more information.
to:
Cortisol (ACTH) Stimulations may be scheduled with RML's PSC center at St. John Medical Center for collections M-F 0730am to 4:00pm at 918-744-3131 x 15531; after 4:00pm may be scheduled with Chemistry department at 981-744-3131 x 15514. Testing is performed Monday through Friday 9:00am to 11am. and requires about 1 hour and 15 minutes of the patient's time.
6/15/18 changed Specimen stability: Ambient 8 hours. Refrigerated 7 Days. Freeze for > 7 Days Stability. to
Stability: Ambient 1 day. Refrigerated 14 days. Frozen 30 days. JK

2022 cpt from 80400, 82533, 80500 to 80400, 82533, 80503 - JK

Service Provided By

Lab Section

Chemistry