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St. John Health System
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Brain Natriuretic Peptide (BNP)

Order Name BRAIN PEP
Test Number: 2015175
Revision Date 04/26/2021
Test Name Methodology LOINC Code
Brain Natriuretic Peptide (BNP)
Chemiluminescence Assay 42637-9 
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 1 mL (0.5 mL) Plasma EDTA (Lavender Top) See Instructions
Alternate 1 2 mL (1 mL) Whole Blood EDTA (Lavender Top) See Instructions
Instructions Separate plasma from cells immediately for best stability.
Draw separate tube if other testing needed on lavender tube.
Plasma Stability after separation from cells: Ambient 4 hrs. Refrigerated 1 day. Frozen 3 months. 
Whole Blood Stability: Ambient 4 hours. Refrigerated 1 day.
GENERAL INFORMATION
Testing Schedule Daily 
Expected TAT 1-2 days 
Clinical Use Aka: B-Type Natriuretic Peptide. Used for management and diagnosis of congestive heart failure (CHF). 
CPT Code(s) 83880
Internal Comments JK  2/22/17 per Ryan Hatley, changed spec instructions away from:
Stable at refrigerated temperature for 24 hours on EDTA Whole blood or EDTA Plasma. If testing cannot be performed within 24 hours, centrifuge and separate plasma from cells, then Freeze plasma in a sterile plastic aliquot tube. Testing is performed on EDTA plasma only within 8 hours of thawing.

to: EDTA whole blood stable at refrigerated temperature for 8 hours. EDTA plasma, if immediately separated from cells, stable at refrigerated temperature for 24 hours. If testing cannot be performed within 24 hours, centrifuge and separate plasma from cells, then freeze plasma in a sterile plastic aliquot tube. Testing is performed on EDTA plasma only within 8 hours of thawing.
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7/14/17 updated processing to : 
Separate plasma from cells immediately for best stability.  Ambient=N/A; Refrigerated=24 hours; Freeze if testing cannot be started within 24 hours. - JK
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4/3/18 removed EDTA Whole Blood - JK
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4/18/18 change stab  from  Refrigerated=24 hours  to Refrigerated (whole blood or plasma)=24 hours
6/14/18 change from Ambient=N/A; Refrigerated (whole blood or plasma)=24 hours; Freeze if testing cannot be started within 24 hours. to
Stability after separation from cells: Ambient 4 hrs. Refrigerated 1 day. Frozen 3 months.  Whole blood stability: Ambient 4 hours. Refrigerated 1 day. - JK
Service Provided By Labcorp Oklahoma, Inc.
Lab Section Chemistry