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St. John Health System
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Index:

Vitamin B 12 / Folic Acid

Order Name B12 FOLAT
Test Number: 4500850
Revision Date 06/04/2021
Test Name Methodology LOINC Code
Folic Acid (Folate)
Chemiluminescence Assay 2284-8 
Vitamin B12
Chemiluminescence Assay 2132-9 
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 1 mL (0.5) Serum Clot Activator SST Refrigerated
Alternate 1 1 mL (0.5) Plasma Lithium Heparin PST (Light Green Top) Refrigerated
Instructions Allow serum to clot fully, then separate serum and plasma within 2 hours of collection.
Unacceptable: hemolyzed serum or plasma. 
Specimen stability: Ambient 8 hours, Refrigerated 7 days, Frozen 1 month
GENERAL INFORMATION
Testing Schedule Daily 
Expected TAT 1-3 days 
Clinical Use Useful in the investigation of macrocytic anemia and work-up of deficiencies seen in evaluate megaloblastic and macrocytic anemias. Also used for diagnosis and investigation of some neurological disorders; and useful in detecting deficiency of folate, monitor therapy with folate; evaluate alcoholic patients; evaluate cause of increase in serum homocysteine level. 
CPT Code(s) 82607; 82746
Internal Comments 11/5/15 - changed instructions per Mary Pille,
from
Non hemolyzed serum. Specimen stability: Ambient 8 hours. Refrigerated 72 hours.
to
Non hemolyzed serum. Specimen stability: Ambient 8 hours. Refrigerated 72 hours, Frozen 1 week.
It would be preferred that specimen be frozen if not going to be tested within 48 hours of collection.

the Difference in Frozen stability is due to the Folate only has 1 week stability.
6/20/19 update processing
9/20/17 updated SU - JK
7/20/18 added lith hep as alt spec - JK
[(RYAN HATLEY) -- 07/17/19 01:09:00 PM] -
1. Please update Transport Environment to "Refrigerated"
2. Please remove from Instructions "It would be preferred that if testing is not going to be performed within 48 hours of collection that the serum or plasma aliquot be frozen for maximum stability." - JK
Service Provided By Labcorp Oklahoma, Inc.
Lab Section Chemistry