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St. John Health System
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Vitamin B12

Order Name VIT B 12
Test Number: 4500900
Revision Date 06/04/2021
Test Name Methodology LOINC Code
Vitamin B12
Chemiluminescence Assay 2132-9 
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 1 mL (0.5 mL) Serum Clot Activator SST Refrigerated
Alternate 1 1 mL (0.5 mL) Plasma Lithium Heparin PST (Light Green Top) Refrigerated
Instructions Allow serum to clot fully, then separate serum and plasma within 2 hours of collection.
Unacceptable: hemolyzed serum or plasma. 
Specimen stability: Ambient 3 days, Refrigerated 7 days, Frozen 1 month
GENERAL INFORMATION
Testing Schedule Daily 
Expected TAT 1-3 days 
Clinical Use Useful in the investigation of macrocytic anemia and work-up of deficiencies seen in megaloblastic anemias. Also used for diagnosis and investigation of some neurological disorders. 
CPT Code(s) 82607
Internal Comments 9/20/17 updated SU - JK
3/8/18 updated temp to emph that "It would be preferred that specimen be frozen if not going to be tested within 48 hours of collection." - JK
7/20/18 added LI Hep as alternate - JK
6/20/19 update processing instructions
[(RYAN HATLEY) -- 07/17/19 01:11:00 PM]
1. Please update Transport Environment to "Refrigerated"
2. Please remove from Instructions "It would be preferred that if testing is not going to be performed within 48 hours of collection that the serum or plasma aliquot be frozen for maximum stability." - JK
Service Provided By Labcorp Oklahoma, Inc.
Lab Section Chemistry