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Paroxysmal Nocturnal Hemoglobinuria (PNH)(CD55/CD59)

Order Name CD55/CD59
Test Number: 0123875
Revision Date 04/27/2009
Test Name Methodology LOINC Code
Percent Normal RBCs
Flow cytometry  
Percentage PNH Cells
Flow cytometry  
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 5 mL (0.2) Whole Blood EDTA (Lavender Top) Room Temperature
Alternate 1 5 mL (0.2) Whole Blood Sodium Heparin (Green Top / No-Gel) Room Temperature
Instructions Stability: Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable.
GENERAL INFORMATION
Testing Schedule Sun-Sat 
Expected TAT 2-4 Days 
Clinical Use The High Resolution PNH-RBC panel detects the absence of phosphatidylinositol-linked membrane proteins CD55 (DAF-decay accelerating factor) and CD59 (MIRL-membrane inhibitor of reactive lysis) on erythrocytes. The erythrocytes are identified with glycophorin staining. All normal RBCs (99.995-100.000%) express CD55 and CD59 and are reported as % Normal RBCs. The expression of CD55 and CD59 is decreased or absent in a subpopulation of RBCs in patients with paroxysmal nocturnal hemoglobinuria. The estimated percentage of RBCs with decreased expression is reported as % PNH cells. 
CPT Code(s) 88184, 88185
Internal Comments OLD NOTES (PRE JAN 2008)- The expression of CD55 and CD59 is decreased or absent in patients with paroxysmal nocturnal hemoglobinuria (PNH). All normal RBCs (100%) express CD55 and CD59. The estimated percentage of RBCs with decreased expression is reported as percent PNH cells.
Service Provided By Labcorp Oklahoma, Inc.
Lab Section Reference Lab