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BRAF Mutation Analysis (V600E)

Order Name BRAF MUTAT
Test Number: 9100927
Revision Date 09/01/2023
Test Name Methodology LOINC Code
BRAF Mutation Analysis (V600E)
Polymerase Chain Reaction  
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred See Instructions Tissue Paraffin Block Room Temperature
Alternate 1 3 mL (1 mL) Whole Blood EDTA (Lavender Top) Room Temperature
Alternate 2 3 mL (1 mL) Bone Marrow EDTA (Lavender Top) Room Temperature
Instructions Collection Instructions:  Specimens must be received by noon on Friday to receive results by Friday.
Primary:Tissue (formalin-fixed, paraffin-embedded block., Tissue (paraffin-embedded section).
Alternate:: 3mL (1mL) Bone marrow or Whole Blood; Unstained 5 um slides (preferably 5 slides) Lavender top tube (EDTA) Pink top tube (EDTA) Yellow top tube (ACD - A)
Rejection Criteria: Tissue specimens with no tumor, frozen specimens and specimens fixed/processed in alternative fixatives (alcohol Prefer®) or fixatives containing heavy metals are unacceptable.
Specimen Processing Instructions: Paraffin-embedded, formalin-fixed tissue block or unstained 5um slides, preferably 5 slides. If the specimen to be tested is a needle biopsy or contains less than 50% tumor, send 10 unstained 5um slides.
Stabilities/Storage (Collection to initiation of testing): Ambient= Indefinite, Refrigerated= Indefinite, Frozen= Unacceptable.
Shipping Instructions: Ship ambient. Protect Paraffin block from excessive heat by shipping in a cooled container during warm weather.
GENERAL INFORMATION
Testing Schedule Testing Initiated on Monday 
Expected TAT Within 7-14 Days 
Clinical Use The BRAF V600E mutation has been identified in 40% to 60% of malignant melanomas. The majority of BRAF mutations seen in melanoma occur in codon 600; the predominant mutation in this codon is V600E (GTG to GAG). Recent clinical trial data show promising results following treatment of melanoma with the BRAF V600E inhibitor, PLX4032 (RG7204, vemurafenib [ZELBORAF(R)]; Plexxikon/Roche Pharmaceuticals). Eighty-one percent of patients harboring the V600E BRAF mutation had complete or partial tumor regression. This BRAF V600E mutation test can be used to help select melanoma patients who are more likely to respond to treatment with vemurafenib. Vemurafenib is not recommended for use in patients with wild type BRAF. 
Notes This test goes to Tricore TC: MDBRAF
CPT Code(s) (Pre-Authorization Required)
81210

Please submit Pre-Authorization form when the patient has United Healthcare insurance.
Internal Comments 9/9/13 changed test number from Quest version 9100925 to Tricore version 9100927
old 83891, 83898x3, 83894x3, 83892x3, 83909x3, 83904x3, 83912
12/6/22 
SNaPshot Multiplex PCR (primer extension-based method)
Labcorp Test Code: 481030
Notes: Two unstained slides and one matching H&E-stained slide at 5uM. Sample with >4mm2 and greater than or equal to 50% tumor content are preferred; 5 mL FNA, 3mL blood, 1 mL bone marrow.
Specimen Type: FFPE tissue block or slides, lavender-top (EDTA) tube, green-top (sodium heparin) tube, yellow-top (ACD-A) tube, FNA CytoLyt container
Specimen Storage: Maintain specimen at room temperature. If specimen is to be stored prior to shipment, store at 2(degrees)C to 8(degrees)C. Store FFPE block or slides, FNA in CytoLyt container at room temperature.
Special Instructions: Please provide a copy of the pathology report. Direct any questions regarding this test to customer service at 800-345-4363. BRAF testing will be delayed if the pathology report is NOT received.
Specimen Stability: Ambient: Not Available, Refrigerated : Not Available, Frozen: Not Available

9/1/23 removed LCA number 481030 from top.. left in Tricore  Ticket 1010654 Julie asked to add "Performing Labcorp Test Code: MDBRAF" this test does not go to Labcorp- JK
Service Provided By Labcorp Oklahoma, Inc.
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