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Mycobacterium tuberculosis (Respiratory sputum) NAA

Order Name MTB NAA
Test Number: 6060550
Revision Date 01/07/2021
Test Name Methodology LOINC Code
Mycobacterium tuberculosis (Respiratory sputum) NAA
Nucleic Acid Amplification  
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 5mL Bronchial lavage/wash Sterile Screwtop Container Refrigerated
Alternate 1 5mL Sputum Sterile Screwtop Container Refrigerated
Alternate 2 5mL Tracheal lavage/wash Sterile Screwtop Container Refrigerated
Instructions For respiratory specimens only. Early morning collection of sputum is preferred. Collect 5-10mL bronchial lavage/wash, tracheal lavage/wash or sputum in sterile screw top container. Keep refrigerated.
Rejection Criteria: Frozen specimens, specimens from patient previously identified with mycobacterium species within prior 6 months (excluding M. gordonae), specimens extensively bloody, specimens < 2 mL volume, specimens containing fixative or foreign object/food particles, non-pulmonary specimens, samples from patient being treated with anti-tuberculosis drugs(unless < 3 days treatment), or specimens from patients < 18 years old.
GENERAL INFORMATION
Testing Schedule Sun-Sat 
Expected TAT 2-5 days for PCR result 
Notes Expected TAT: 2-5 days for PCR result; specimens negative for MTB NAA test will reflex to culture and be incubated 42 days before a final negative report is issued.

Performed at the Oklahoma State Department of Health Laboratory
  • A positive result infers the presence of M. tuberculosis Complex DNA, not viable organisms. The assay does not differentiate between species within the M. tuberculosis Complex.
  • A negative result does not exclude the possibility of M. tuberculosis Complex infection; detection is dependent on appropriate specimen collection and handling, absence of inhibitors, and sufficient levels of organisms. Since the assay cannot detect non-tuberculosis mycobacterial species, culture should be used to determine if non-tuberculosis mycobacteria are present.
  • An indeterminate result may be due to improper processing of sample, or presence of a PCR inhibitor; collection of a fresh specimen for testing is recommended.
  • This assay is not suitable for monitoring therapeutic efficacy.
CPT Code(s) N/A
Internal Comments 5/30/18 added instructions and notes - JK
7-9-18 removed min volume of 2mL - JK
1/7/21 updated the long name- JK
Service Provided By Labcorp Oklahoma, Inc.
Lab Section Microbiology