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St. John Health System
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HIV Antigen/Antibody Screen, 4th Gen

Order Name HIV AG/AB
Test Number: 3609705
Revision Date 01/04/2024
Test Name Methodology LOINC Code
HIV Antigen/Antibody Screen, 4th Gen
Immunoassay (IA) 56888-1 
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 3.0mL (2.0mL) Serum Clot Activator SST Refrigerated
Alternate 1 3.0mL (2.0mL) Plasma Lithium Heparin PST (Light Green Top) Refrigerated
Instructions Please submit original, un-opened serum separator tube.
Allow to 15-20 minutes to clot then Centrifuge - Do Not Open!
Stability: Ambient 3 days, refrigerated 7 days, Frozen >7 days

Keep Specimens Refrigerated for best stability.
GENERAL INFORMATION
Testing Schedule Mon - Fri 
Expected TAT 1-3 days 
Notes HIV-1/2 Antibody Differentiation will be performed at an additional charge (CPT code(s): 86701, 86702).
Reflex Order code: HIV-1/2 Antibody Differentiation [3609707]
Reflex Result code: HIV 1 Antibody [3609713]  LOINC: 68961-2
Reflex Result code: HIV 2 Antibody [3609717]  LOINC: 81641-3

If HIV-1/2 Antibody Differentiation is Indeterminate or Negative, HIV-1/HIV-2 Qualitative RNA will be performed
at an additional charge (CPT code(s): 87538, 87535).
Reflex Order code: HIV-1/HIV-2 Qualitative RNA [5194321]
Reflex Result code: HIV-1 Qualitative RNA [5194322] LOINC: 25835-0
Reflex Result code: HIV-2 Qualitative RNA [5194323] LOINC: 69353-1
CPT Code(s) 87389 (See possible Reflex codes in Notes Section.)
 
Internal Comments 12/5/2016 - Added to RML Online - JLS
4/24/17 updated for 4/26/17 hiv cutover from 3rd gen test, JK
9/20/17 updated su - JK
12/26/17 removed tiger - JK
5/22/18 old collection requirements removed- JK
Please submit TWO UN-OPENED Serum Separator tubes.
Allow to 15-20 minutes to clot then Centrifuge - Do Not Open!
Tube 1: Used for Initial testing
Tube 2: Used for Confirmation testing.
Specimen Stability: Room temperature: 72 hours, Refrigerated: 5 days, Frozen: 30 days.

Keep Specimens Refrigerated for best stability.
If testing cannot be started within 5 Days of collection, please submit the following:
One 2mL(1mL) Frozen Serum Aliquot tube - and -
One Un-Opened Clot Activator SST, Spun down and Frozen.

6/15/18 changed
Specimen Stability: Room temperature: 72 hours, Refrigerated: 5 days, Frozen: 30 days. to
Stability: Ambient 2 days. Refrigerated 5 days. Frozen 30 days. JK

10/122 removed clinical use
RML has transitioned to a 4th generation HIV assay from the previous 3rd generation HIV 1/O/2 Antibody test. Any interfaced order for the 3rd generation HIV test will have the 4th generation HIV order added on to the patient and the 3rd generation order will be canceled with a cancel reason of “duplicate”. The results for these reflex orders will be delivered by paper, either by courier or fax based depending on the scenario.
Service Provided By Labcorp Oklahoma, Inc.
Lab Section Chemistry