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St. John Health System
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Index:

T Cell Fx-Complete

Order Name T Cell Fx-Comp
Test Number: 5194442
Revision Date 12/12/2022
Test Name Methodology LOINC Code
T Cell Fx-Complete
Flow cytometry  
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 8 mL Whole Blood Sodium Heparin (Green Top / No-Gel) Room Temperature
Instructions Must be drawn between 12-2PM CST Monday through Thursday and sent via STAT courier to LabCorp Oklahoma Core Lab.
Specimen Type: Green-top (sodium heparin) tube
Specimen Storage: Room temperature
Specimen Collection: Specimens must arrive at Viracor-Eurofins within 24 hours collection
Special Instructions: Testing referred to Eurofins Viracor LLC TC#403066
Specimen Stability: Ambient: 24 hours, Refrigerated : Not Available, Frozen: Not Available
GENERAL INFORMATION
Expected TAT 5 - 6 days 
Clinical Use The test measures the T cell responses to antigens of common infections as well as to mitogens, allowing detection of deficiencies in this arm of the immune response. Patients with primary immunodeficiency may have deficits in the acquired T-cell response. It is useful for the following types of patients: Immune deficiency, Recurrent infection, Evaluation of cell-mediated immunity and monitoring immunosuppression (e.g. Transplant patients). 
Notes Labcorp Test Code: 831367
(Testing performed at Viracor-Eurofins)
Methodology: Peripheral blood mononuclear cells are stimulated with mitogens or antigens or left unstimulated as a negative control and incubated at 37ºC. During the incubation Brefeldin A is added, causing the interferon (IFN)-gamma to be retained inside the cell. Following the stimulation phase, cells are recovered, stained for surface markers (CD45, CD3, and CD69 and intracellular IFN-gamma, and analyzed by flow cytometry. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.
CPT Code(s) 86353x7
Service Provided By Labcorp Oklahoma, Inc.
Lab Section Reference Lab