PIK3CA Mutation Analysis
Order Name
PIK3CA Mutat
Test Number: 6907417
Revision Date 12/12/2022
Test Number: 6907417
Revision Date 12/12/2022
| Test Name | Methodology | LOINC Code |
|---|---|---|
|
PIK3CA Mutation Analysis
|
Polymerase Chain Reaction/Sequencing | 60034-6 |
| SPECIMEN REQUIREMENTS | ||||
|---|---|---|---|---|
| Specimen | Specimen Volume (min) | Specimen Type | Specimen Container | Transport Environment |
| Preferred | 1 Block | Tissue | Paraffin Block | Room Temperature |
| Alternate 1 | See Instructions | Tissue | See Instructions | Room Temperature |
| Instructions | Notes: 3 unstained slides at 4-5m and one matching H&E-stained slide or 4 unstained slides. Resection or Surgical Biopsies require > or =10% tumor content. Core Needle Biopsies require a minimum of > or = 20 mm2 tumor area total available for extraction. Minimum Specimen Type: FFPE block or slides Specimen Storage: Maintain blocks/slides at room temperature. Specimen Collection: Ship specimen at room temperature. Please direct any questions regarding this test to customer service at 800-345-4363. Special Instructions: Please provide a copy of the pathology report. Testing will be delayed if the pathology report is NOT received. Please direct any questions regarding this test to customer service at 800-345-4363. Specimen Stability: Ambient: Not Available, Refrigerated : Not Available, Frozen: Not Available |
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| GENERAL INFORMATION | |
|---|---|
| Expected TAT | 7 days from setup |
| Clinical Use | PIK3CA Mutation Analysis - PIK3CA mutation has been associated with poor prognosis in endometrial, breast and colorectal cancers. Mutations in exons 9 and 20 of PIK3CA ave also been associated with resistance to cetuximab therapy in patients with colorectal cancer. |
| Notes | The therascreen PIK3CA RGQ PCR Kit is a real-time qualitative PCR in vitro diagnostic test, performed on the Rotor-Gene Q MDx (US) instrument. It uses allele refractory mutation system (ARMS) primers, hydrolysis probes and PCR clamp technologies to detect 11 mutations in exons 7, 9 and 20 of the PIK3CA oncogene against a background of wild-type gDNA. Labcorp Test Code: 485113 |
| CPT Code(s) | 88381, 0155U |
| Service Provided By |  |
| Lab Section | Reference Lab |